TideCell Cell Harvesting System (TCCHS) is specially designed for TideCell HD Cell Culture systems for cell recovery from thecultured Matrix Vessel. The TCCHS is a powerful tool to harvest and transfer viable cells from large scale TideCell systems in a closed and automatic system. It can also be applied to harvest cell mass for biological production and any related applications. Up to 1011 cells can be harvested within one to three hours operation.
TCCHS is a closed, automated system for harvesting attached, viable cells from 2L to 20L TideCell® bioreactors. Being a closed system, it is perfect for harvesting seed cells for scaling up without having to worry about contamination. The system has a recovery rate of up to 90%. TCCHS utilizes the same as conventional cell harvest concept by enzymatic treatment that could detach cells from attached substrates. It uses conventional solutions and processes for cell harvesting and automates it.
- Enzymatic Detachment – Pump in trypsin or equivalent solution to detach cells from the matrix.
- Inhibitor – Pump in inhibitors to stop the enzymatic reaction.
- Detachment – Automatically shake the matrix vessel at a preset force and duration.
- Harvesting – Pump in culture media while shaking and rolling the matrix vessel to loosen cells from the matrix. Pump out the media with suspended cells to harvest tank.